ELISpot Flex: Mouse IFN-α (HRP)

ELISpot Flex: Mouse IFN-α (HRP)

3326-2H

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT14A/104/113
Detection mAbMT9L/24A/44A, biotin
Enzyme conjugateStreptavidin-HRP

In stock

Delivery 4-9 business days

Shipping $0

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Complementary products
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ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IFN-α (pan specific) using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse IFN-α (HRP)
ApplicationELISpot
AnalyteIFN-α pan
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of mouse IFN-α (pan specific). Subtypes 1, 2, 4, 5, 6T, 7/10 (129/Sv), 8/6, 9, 11, 12, 13, 14, 15 (A), and B are detected.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (500,000 cells/well) were incubated overnight in the absence or presence of 1µM ODN 1585 and the number of cells secreting IFN-α was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IFN-α pan

Analyte description

Interferon-α and interferon-β are type I interferons. These antiviral cytokines are mainly produced by virus-infected cells after sensing the presence of danger signals such as viral DNA or RNA. IFN-α and IFN-β act through the same receptor and initiate an anti-viral response, affect immune regulation, T cell responses, and also anti-tumor effects have been shown. A key producer of type I interferons is the plasmacytoid dendritic cell. Additionally, IFN-α is secreted by lymphocytes, macrophages, fibroblasts, and dendritic cells. 

In humans, interferon-α has 13 subtypes: IFN-α 1, 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21.

In mice, there are 14 IFN-α subtypes: 1, 2, 4, 5, 6T, 7/10, 8/6, 9, 11, 12, 13, 14, 15 (A), and B. There are also different allelic forms of mouse IFN-α subtypes 1, 7/10, 8/6, and 11 in mouse strains 129/Sv and C57BL/6.

Alternative namesInterferon-α, Interferon-alpha, IFN-α, IFN-alpha, IFN-a, IFNA